EnglishDTU.dkIndeksKontaktTelefonbogAlumnenetværkPortalen
Center for Microbial Biotechnology (CMB) > Publikationer 2012
Publikationer 2011
Publikationer 2010
Publikationer 2009
Publikationer 2008
Publikationer 2007
Publikationer 2006
Publikationer 2005
Publikationer 2004
Publikationer 2003
Titel: Production of novel fusarielins by ectopic activation of the polyketide synthase 9 cluster in Fusarium graminearum
Type: Journal articleJournal article
Person(er):
Forfatter:  Sørensen, Jens Laurids
Faculty of Science and Technology, Aarhus University

Forfatter:  Hansen, Frederik Teilfeldt
Institut for Molekylærbiologi og Genetik

Forfatter:  Sondergaard, Teis Esben
Faculty of Science and Technology, Aarhus University

Forfatter:  Staerk, Dan
Faculty of Life Sciences, University of Copenhagen

Forfatter:  Lee, T. Verne
School of Biological Sciences, University of Auckland

Forfatter:  Wimmer, Reinhard
Chemistry and Environmental Engineering, Aalborg University

Forfatter:  Klitgaard, Louise Graabæk
Faculty of Science and Technology, Aarhus University

Forfatter:  Purup, Stig
Faculty of Science and Technology, Aarhus University

Forfatter:  Giese, Henriette
Faculty of Science and Technology, Aarhus University

Forfatter:  Frandsen, Rasmus John Normand (Cwisno: 53158)
Department of Systems Biology, Technical University of Denmark, Søltofts Plads 223, DK‐2800 Kgs. Lyngby, Denmark.
Email:

Uddrag: Like many other filamentous fungi, Fusarium graminearum has the genetic potential to produce a vast array of unknown secondary metabolites. A promising approach to determine the nature of these is to activate silent secondary metabolite gene clusters through constitutive expression of cluster specific transcription factors. We have developed a system in which an expression cassette containing the transcription factor from the targeted PKS cluster disrupts the production of the red mycelium pigment aurofusarin. This aids with identification of mutants as they appear as white colonies and metabolite analyses where aurofusarin and its intermediates are normally among the most abundant compounds. The system was used for constitutive expression of the local transcription factor from the PKS9 cluster (renamed FSL) leading to production of three novel fusarielins, F, G and H. This group of compounds has not previously been reported from F. graminearum or linked to a biosynthetic gene in any fungal species. The toxicity of the three novel fusarielins was examined against colorectal cancer cell lines where fusarielin H was more potent than fusarielin F and G.
Publiceret: in journal: Environmental Microbiology (ISSN: 1462-2912) (DOI: http://dx.doi.org/10.1111/j.1462-2920.2011.02696.x), vol: 14, issue: 5, pages: 1159-1170, 2012
DOI:
Se publikationen i DTU Orbit Se publikationen i DTU Orbit

Top
Søltofts PladsBygn. 221/2232800 Kgs. LyngbyTlf. 4525 2694CVR-nr. 30 06 09 46EAN-nr. 57 98 00 04 30 273 (stednr. 2732)
Cookies